Monitoring Lys-tRNALys phosphatidylglycerol transferase activity
نویسندگان
چکیده
منابع مشابه
Metabolism of Phosphatidylglycerol and Lysyl Phosphatidylglycerol in Staphylococcus aureus.
The metabolism of phosphatidylglycerol and lysyl phosphatidylglycerol was studied in Staphylococcus aureus under four conditions: growing at pH 7.0 and 5.2, and not growing (resting) at pH 7.0 and 5.2. Measurements of the amounts of phosphatidylglycerol and lysyl phosphatidylglycerol, as well as labeling and pulsechase experiments, revealed that the phosphate group of the former and the lysyl g...
متن کاملThe biosynthesis of phosphatidylglycerol.
Phosphatidylglycerol has been shown to be a constituent of the lipids of both plant and animal tissues by Benson and Maruo (1) and Strickland and Benson (2). It has also been detected in bacteria (3,4). Benson and Maruo (1) have called attention to the rapid rate of metabolism of phosphatidylglycerol in plants, and Kanfer and Kennedy 1 have obtained evidence that this phosphatide is a metabolic...
متن کاملPhosphatidylglycerol and Related Lipids
Phosphatidylglycerol is found in almost all bacterial types. For example, Escherichia coli, a widely studied organism, has up to 20% of phosphatidylglycerol in its membranes (phosphatidylethanolamine makes up much of the rest with a little cardiolipin). In many bacteria, the diacyl form of the lipid predominates, but in others the alkylacyland alkenylacyl forms are more abundant. Phosphatidylgl...
متن کاملThe Binding of Phosphatidylglycerol Liposomes
Previous work has shown that intravenous administration of phosphatidylglycercol (PG) containing liposomes to rats results in a rapid transient decline in platelet count (1). Here the interactions of PG liposomes with rat platelets in vitro have been examined with the aim of characterizing factors associated with the decline. It is shown that PG liposomes induce formation of rat (but not human)...
متن کاملCytoplasmic Particles and Aminoacyl Transferase I Activity
It has been possible to show by electron microscopy of samples selected from sucrose gradients that particles of specific size and shape are present in supernatant fluids derived from nucleated animal and plant cells, but not in extracts from Escherichia coli. Aminoacyl transferase I activity in these same gradients sediments in two peaks representing material of approximately 5-7S and 18-20S. ...
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ژورنال
عنوان ژورنال: Methods
سال: 2008
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2007.09.002